Multicentric lymphosarcoma in a dog

A 10 year old male Dalmatian dog carcass on necropsy exhibited enlarged of peripheral lymph nodes, hepatomegaly, splenomegaly with three grayish white nodules of 7x5x3, 3x3x1 and 2x1x1 cm in size on the ventral surface of spleen, enlarged kidneys with grayish white foci (1x0.5 cm size) on the cortex, enlarged mesenteric lymph nodes, pallor bone marrow and congested stomach and intestines. Fine needle aspiration biopsy of peripheral lymph nodes showed large lymphoblast cells with occasional mitotic figures. Ultrasonography revealed splenomegaly and hepatomegaly. Cytologically, it was classified as lymphocytic diffuse type, poorly differentiated lymphosarcoma. Biochemically, hypercalcaemia was recorded in the serum. Microscopically, solid, dense sheets of lymphoblast cells were seen with scanty cytoplasm.