Indian Journal of Veterinary Pathology
Open Access
  • Year: 2011
  • Volume: 35
  • Issue: 2

Induction of skin tumour using DMBA in wistar Rat and histopathological evaluation

  • Author:
  • R.S. Rajmani4, J. Doley4, P.K. Singh4, Ravi Kumar4, R. Singh1,4, R. Barathidasan2,4, Pawan Kumar2,4, P.C. Verma3,4, A.K. Tiwari*,4
  • Total Page Count: 4
  • Published Online: Dec 1, 2011
  • Page Number: 217 to 220

1Pathology Laboratory, CADRAD, Izatnagar-243122 (U.P.)

2Division of Pathology, Indian Veterinary Research Institute, Izatnagar-243122 (U.P.)

3Division of Standardization, Indian Veterinary Research Institute, Izatnagar-243122 (U.P.)

4Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122 (U.P.)

*Corresponding author: email: aktiwari@yahoo.com

Abstract

In the present study, skin tumours in male wistar rats were induced experimentally by employing 1% DMBA (7, 12 -Dimethylbenz (a) anthracene) (2.5mg DMBA in 0.25ml of acetone to each rat) for evaluation and validation of new anticancer therapeutic modules. All the rats developed squamous cell carcinomas in 20 to 25 weeks as confirmed by histopathology, mitotic index, AgNOR staining and PCNA staining of tumour tissues.

Keywords

AgNOR, DMBA, PCNA mitotic index, Squamous cell carcinoma, Tumour