Indian Journal of Veterinary Pathology
Open Access
  • Year: 2013
  • Volume: 37
  • Issue: 1

Comparative evaluation of Ziehl Neelsen staining and immunohistochemical detection of M. avium subsp. paratuberculosis using polyclonal antibodies against M. tuberculosis in bovine tissues

  • Author:
  • Julius Boniface Okuni2,, Lonzy Ojok2, Panagiota Tsaousi1,2, Panayiotis Loukopoulos1
  • Total Page Count: 4
  • Published Online: Jun 1, 2013
  • Page Number: 64 to 67

1Laboratory of Pathology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece

2Department of Veterinary Pathology, School of Veterinary Medicine, Makerere University, P.O. Box 7062, Kampala, Uganda

*Corresponding author: email: jbok@vetmed.mak.ac.ug

Abstract

Immunohistochemical (IHC) staining of acid-fast bacteria has been reported to be superior to Ziehl Neelsen (ZN) staining in the detection of mycobacteria in tissues. The potential use of commercial antibodies against M. tuberculosis has been evaluated for the detection of MAP infection in bovine tissues and compared it to the more commonly used ZN staining. From this study, commercial anti-M. tuberculosis antibodies may be used in the detection of MAP in animal tissues as a screening test as compared to ZN staining, in the absence of lesions indicative of other mycobacterial infections, in lieu of Ziehl Neelsen staining.

Keywords

Bovine, Immunohistochemistry, M. avium paratuberculosis, M. tuberculosis