Expression of certain cancer biomarkers in urinary bladder tumors/lesions of cattle and buffaloes

A total of 20 urinary bladder (UB) samples were studied for presence of bovine papilloma virus (BPV) and evaluated the expression of biomarkers viz. PCNA, Ki67, c-Myc and c-ErbB2 in the tumours/lesions of cattle and buffaloes by immunohistochemistry (IHC). PCR revealed the presence of BPV-2 DNA in 11 UB samples. Among BPV-2 positive UB samples, 2 also showed the presence of BPV-1 DNA. IHC of UBT (papillary and non infiltrative TCC, papillary and infiltrative TCC, hemangiosarcoma, adenoma etc.) revealed that PCNA, Ki67 and c-Mycwere potent markers for assessing the proliferation status of cells in these neoplasms. Expression of the c-ErbB2 was observed mainly in the neoplastic cells of glandular and papillary areas of the urinary bladder tumours.