Immunohistochemical evaluation of antidiabetic effect of Momordica charantia extract in cow urine in streptozotocin-induced diabetic rat model

Momordica charantia, also referred to as bitter gourd or karela, is a member of the Cucurbitaceae family and is commonly used as a traditional remedy for diabetes in India, Asia, Africa and South America. According to Sushruta Samhita and Ayurveda Samgraha for medicinal and other purposes cow urine is beneficial and better than of all other animals. The present study was undertaken to evaluate the antidiabetic effect of Momordica charantia extract and cow urine in streptozotocin-induced diabetic rat model using immunohistochemistry for a period of 45 days. The alcoholic extract of Momordica charantia was administered orally at the dose rate of 200 mg/kg body weight in cow urine and compared with standard oral hypoglycaemic drug, glibenclamide. Immunohistochemistry revealed improvement in the insulin secretion in various treatment groups with Momordica charantia 200 mg/kg body weight in cow urine being more effective compared to Momordica charantia 200 mg/kg body weight in distilled water.