1Department of Veterinary Microbiology, COVAS, Udaipur, Rajasthan;
2Division of Animal Genetics and Breeding, IVRI, Izatnagar, Uttar Pradesh-243122
3Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute, Izatnagar;
*Corresponding author: e-mail: chandanguptaivri@gmail.com
Single intradermal cervical tuberculin skin testing (TST) is only available diagnostic test for detection of tuberculosis in bovines in India. TST may miss some positive tuberculosis cases due to its limited sensitivity. Present study was aimed to evaluate different diagnostic tests (ELISA and PCR) for detection of tuberculosis in cattle. A total of 123 randomly selected cattle were examined for tuberculosis with TST, ELISA and PCR. Detection rate of tuberculosis by TST, ELISA and PCR were 8.94%, 13.82% and 5.69% respectively. This detection rate could be increased up to 18.69% by using multiple diagnostic tests serially. ELISA and PCR could detect the disease condition in advance stage, failing to detect by TST. Use of multiple diagnostic tests may minimize chance of missing the positive cases.
Tuberculosis, Tuberculin skin test, ELISA, PCR
