1Department of Veterinary Public Health & Epidemiology, College of Veterinary Science and Animal Husbandry, Central Agricultural University, Aizawl-796014 (Mizoram);
2Department of Veterinary Clinical Medicine, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022 (Assam)
3Department of Veterinary Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022 (Assam)
*Corresponding author: e-mail: drdevajani@gmail.com
A total of 178 faecal samples from diarrhoeic dogs were subjected to sandwich ELISA for detection of canine parvovirus (CPV) and 45 samples were found positive. Fifteen random faecal samples positive in sandwich ELISA were inoculated in CRFK cell line for isolation of the virus and 8 (53.33%), 10 (66.67%) and 11 (73.33%) samples were positive for CPV in first, second and third passage, respectively. All the 11 cell culture isolates did not show any cytopathic effect (CPE) in cell monolayer up to third passage.
Cell culture, CPV, CRFK, Diarrhoeic dogs, Sandwich ELISA
