Indian Journal of Veterinary Pathology
Open Access
  • Year: 2016
  • Volume: 40
  • Issue: 3

Serological study for detection of foot-and-mouth disease virus activity in breeding bulls of an elite herd of North India

  • Author:
  • M. Rout3,, M.R. Senapati1,3, J.K. Mohapatra3, T.K. Mohanty2,3
  • Total Page Count: 3
  • Published Online: Sep 1, 2016
  • Page Number: 254 to 256

1Agro Polytechnic Center, Orissa University of Agriculture and Technology, Chiplima, Sambalpur (Odisha)

2Artificial Breeding Research Center, ICAR-National Dairy Research Institute, Karnal-132 001 (Haryana)

3ICAR-Project Directorate on Foot and Mouth Disease, IVRI Campus, Mukteswar-263138, Nainital (Uttarakhand)

*Corresponding author: e-mail: drmrout@gmail.com

Abstract

A serological study using 3AB nonstructural protein ELISA and liquid phase blocking ELISA to assess foot-and-mouth disease virus (FMDV) non-structural and structural protein antibodies (NSP- and SP-Abs) was performed through sampling from 103 cattle and 37 buffalo bulls of an elite herd of north India. A total of 53 (51.46%) cattle bulls and 14 (37.84%) buffalo bulls were found positive for NSP-Ab indicating an exposure to FMDV. The proportion of animals showing ≥1.8 log10titre against all three serotypes in the vaccine varied from 58.25% for cattle bulls and 48.64% for buffalo bulls indicating poor vaccinal herd immunity, which assumably might have made the animals susceptible to virus exposure in the farm.

Keywords

Breeding bull, FMDV, Foot-and-mouth disease, Liquid phase blocking ELISA, 3AB NSP ELISA