A dose response study of monosodium iodoacetate induced femorotibial osteoarthritis in Wistar rats

In the present study,18 female rats were randomly divided into 3 different groups. Each group consisted of 6 female rats. The right femorotibial joint of rats of the group I, II, III received 1, 3 and 5 mg monosodium iodoacetate (MIA), respectively at dose volume of 50 il/joint. The left femorotibial joint of rats of the group I, II, III received 50 il normal saline and served as control. Three rats from each group were euthanized on day 15 and 30 post injection.The clinical sign and histopathologic changes were clearly dose dependent. 5 mg MIA/joint produced marked joint swelling, lameness and lesions viz., degeneration and necrosis of chondrocytes, atrophy of articular cartilage, exposure of subchondral bone, fibrosis in marrow cavity and chondro-osteophyte formation on day 15 and 30. The rats of 3 mg MIA/joint showed similar clinical signs and lesions of milder severity. In 3 mg MIA/joint, chondro-osteophyte formation was seen on day 30. In 1 mg MIA/joint showed mild swelling, lameness and minimal necrosis of chondrocytes as compared to 3 and 5 mg groups. On basis of above findings it is concluded that 3 mg MIA/joint produced ideal osteoarthritisrat model.