Pathological diagnosis of an outbreak of pasteurellosis in buffaloes of Vidharbha region in India

An outbreak of pasteurellosis with 15% mortality was recorded in buffaloes in central India. The presence of Pasteurella multocida in samples collected from dead buffaloes was detected by smear examination and isolation, and later by P. multocida specific polymerase chain reaction (PM-PCR). P. multocida was detected in all the samples. All the isolates were positive by PM-PCR, capsular type B (CAPB) and toxigenic gene (toxA). Histopathologically, sections from lung revealed severe pneumonia and deposition of fibrin in cardiac muscles. The PCR assay used in this study has been shown to be useful diagnostic tool for P. multocida detection.