1department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India
2department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India
3Department of Veterinary Public Health, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India
4Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India
*Address for Correspondence: Dr. Madhu Swamy, Professor and Head, Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh, India; E-mail: drmadhuswamy1@gmail.com
Online published on 12 October, 2020.
The present study was conducted on 200 diarrheic fecal samples collected from cattle and buffalo calves, up to the age of 90 days in Jabalpur region. The samples were subjected to antigen ELISA and RNA-PAGE, for the detection of bovine rotavirus A. The study showed the occurenceof rotavirus as 10% (20/200) and 11% (22/200) by ELISA and RNA-PAGE, respectively. RNA-PAGE revealed the typical migration pattern of 4:2:3:2 of bovine rotavirus A. All the samples were further screened by RT-PCR using primers for the VP6 gene of bovine rotavirus and all the 22 samples positive by ELISA and/or PAGE yielded an amplification product of size of 227bp (100%). The study suggests that RNA-PAGE might be considered as better and cost effective technique for the detection of rotavirus as compared to ELISA.
ELISA, RNA-PAGE, Rotavirus A, VP6
