1Department of Veterinary Pathology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh
2Veterinary Assistant Surgeon, VD, Bapirajugudem, Andhra Pradesh
3Department of Veterinary Public Health & Epidemiology, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh, India
4Veterinary Polytechnic, Venkataramannagudem, Andhra Pradesh, India
*Address for Correspondence Ch. Sudha Rani Chowdary, Assistant Professor, Department of Veterinary Pathology, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, India, E-mail: drsudha84@gmail.com
Online published on 29 August, 2023.
The prevalence of Extended-spectrum β-lactamase (ESBL) producing Escherichia coli has increased significantly during the last decade which represents a major risk both to poultry production and to human health. The present study deals with the genetic detection of ESBL producing E. coli isolates associated with colibacillosis in chickens suspected at necropsy. A total of 16 E.coli isolates that were confirmed phenotypically as ESBL producers were subjected to multiplex PCR for amplification of six β-lactamase genes (blaTEM, blaSHV, blaOXA, blaCTX-M1, blaCTX-M2 and blaCTX-M9). The PCR results revealed that the 16 E.coli isolates carried one or more β-lactamase genes. Of all the β-lactamase genes studied, the majority of the isolates carried blaTEM gene, followed by blaCTX-M2, blaCTX-M1, blaCTX-M9, blaSHV and blaOXA either singly or in combination. The results indicate the existence of ESBL genes in avian pathogenic E.coli isolates of chicken with blaTEM and blaCTX-M2 being the two β-lactamases detected predominantly.
Betalactamases, Colibacillosis, E. coli, ESBL genes, Genotypic detection, Multiplex PCR
