<p>Female genital tract of cattle has considerable biological and economic importance. Genital organ disorders are an important cause of infertility and sterility. Infertility is a major problem in dairy cattle production to the point that it is becoming increasingly apparent, that high yielding cows were unable to conceive under optimum conditions. This negative association between production and fertility has been observed in several dairy breeds. Among the genital organs, ovary plays a major role in the production of reproductive hormones thereby influencing fertility. The pathological conditions of ovary were considered as one of the major causes for infertility. To carry out the present research work, ovaries were collected from 100 cattle and examined to study the prevalence of ovarian disorders in cattle. Out of 100 cases examined <italic>(77%)</italic> cases showed lesions in the ovary and the remaining (23%) were normal. The majority of ovarian tissue samples showed minimal gross lesions that included cysts of varied morphology on the ovarian surface, mild to moderate increase in the size of the ovary. Impression smears revealed abnormal cellular patterns in follicular cysts, luteal cysts and ovarian tumours which were further confirmed by histopathological studies. Based on histopathological studies the identified lesions were oophoritis which was the most common condition and it was recorded in fourteen cases of ovaries (18.18%) followed by follicular cyst in ten cases (12.98%), adenocarcinoma in ten cases (12.98%), granulosa cell tumour in seven cases (9.09%), hemangiosarcoma in seven cases (9.09%), folliculoids in four cases (5.19%), clear cell carcinoma in four cases (5.19%), luteal cyst in four cases (5.19%), scirrhous adenocarcinoma in two cases (2.59%), epoophoron in two cases (2.59%), sclerosed ovary in two cases (2.59%), embedded corpus luteum in two cases (2.59%), cystadenoma of rete ovarii in two cases (2.59%), cavernous hemangioma in one case (1.29%), smooth ovaries in one case (1.29%), dysgerminoma in one case (1.29%), fibrosarcoma in one case (1.29%), hypoplastic ovaries on case (1.29%) and lymphosarcoma in one case (1.29%). Major deviation from normal histology was identified in ovarian tumours. Histopathological examination of adenocarcinoma, clear cell carcinoma, scirrhous adenocarcinoma, hemangioma, hemangiosarcomas, lymphosarcoma, fibrosarcoma, granulosa cell tumours and dysgerminoma revealed characteristic benign and malignant features. The mean AgNOR count for all of the individual tumours varied from 2.74 to 6.24. Fibrosarcoma showed the highest AgNOR count (6.24±1.14). Expression of VEGF (Vascular endothelial growth factor), cytokeratin, vimentin and inhibin was detected in the ovarian neoplasms. Hence, it was concluded that cytopathology can act as an early diagnostic tool for detection of different pathological conditions of the ovaries of cattle which can be further confirmed by detailed histopathological studies. Immunohistochemical examination acts as an aid in confirming epithelial mesenchymal differentiation, angiogenesis and tumour specific antigens.</p></sec></body></article></article></body></html>

ijvp

Indian Journal of Veterinary Pathology

0250-47580973-970X

Indian Association Of Veterinary Pathologists

ijvp-47-2-017

ABSTRACTS OF DISSERTATIONS OR ARTICLES

Pathomorphological and immunohistochemical studies on ovary of cattle

Sravya

MVSc/2023 Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh - 517502

Online published on 29 August, 2023.

2962023

472186186

2023

IndianJournals.com

<p>Female genital tract of cattle has considerable biological and economic importance. Genital organ disorders are an important cause of infertility and sterility. Infertility is a major problem in dairy cattle production to the point that it is becoming increasingly apparent, that high yielding cows were unable to conceive under optimum conditions. This negative association between production and fertility has been observed in several dairy breeds. Among the genital organs, ovary plays a major role in the production of reproductive hormones thereby influencing fertility. The pathological conditions of ovary were considered as one of the major causes for infertility. To carry out the present research work, ovaries were collected from 100 cattle and examined to study the prevalence of ovarian disorders in cattle. Out of 100 cases examined <italic>(77%)</italic> cases showed lesions in the ovary and the remaining (23%) were normal. The majority of ovarian tissue samples showed minimal gross lesions that included cysts of varied morphology on the ovarian surface, mild to moderate increase in the size of the ovary. Impression smears revealed abnormal cellular patterns in follicular cysts, luteal cysts and ovarian tumours which were further confirmed by histopathological studies. Based on histopathological studies the identified lesions were oophoritis which was the most common condition and it was recorded in fourteen cases of ovaries (18.18%) followed by follicular cyst in ten cases (12.98%), adenocarcinoma in ten cases (12.98%), granulosa cell tumour in seven cases (9.09%), hemangiosarcoma in seven cases (9.09%), folliculoids in four cases (5.19%), clear cell carcinoma in four cases (5.19%), luteal cyst in four cases (5.19%), scirrhous adenocarcinoma in two cases (2.59%), epoophoron in two cases (2.59%), sclerosed ovary in two cases (2.59%), embedded corpus luteum in two cases (2.59%), cystadenoma of rete ovarii in two cases (2.59%), cavernous hemangioma in one case (1.29%), smooth ovaries in one case (1.29%), dysgerminoma in one case (1.29%), fibrosarcoma in one case (1.29%), hypoplastic ovaries on case (1.29%) and lymphosarcoma in one case (1.29%). Major deviation from normal histology was identified in ovarian tumours. Histopathological examination of adenocarcinoma, clear cell carcinoma, scirrhous adenocarcinoma, hemangioma, hemangiosarcomas, lymphosarcoma, fibrosarcoma, granulosa cell tumours and dysgerminoma revealed characteristic benign and malignant features. The mean AgNOR count for all of the individual tumours varied from 2.74 to 6.24. Fibrosarcoma showed the highest AgNOR count (6.24±1.14). Expression of VEGF (Vascular endothelial growth factor), cytokeratin, vimentin and inhibin was detected in the ovarian neoplasms. Hence, it was concluded that cytopathology can act as an early diagnostic tool for detection of different pathological conditions of the ovaries of cattle which can be further confirmed by detailed histopathological studies. Immunohistochemical examination acts as an aid in confirming epithelial mesenchymal differentiation, angiogenesis and tumour specific antigens.</p></sec></body></article></article></body></html>