1Department of Veterinary Pathology, Veterinary College and Research Institute, Namakkal-637 002, TANUVAS
2Poultry Disease Diagnosis and Surveillance Laboratory, Madras Veterinary College, Chennai
3Department of Animal Biotechnology, Madras Veterinary College, Chennai
*Address for Correspondence Sedeneinuo Suohu, Ph.D Scholar, Department of Veterinary Pathology, Veterinary College and Research Institute, Namakkal-637 002, India, E-mail: iamsedesuohu@gmail.com
Online Published on 2 January, 2024.
Chicken Anaemia Virus (CAV) is one of the emerging pathogens of poultry which causes immunosuppression in the affected birds and can spread by horizontal as well as vertical route. In the present study, chicken embryonated eggs were inoculated with CAV virus and gross, histopathological examination and molecular detection were carried out to assess the immunosup-pressive effect of CAV in chicken embryo. The bone marrow of CAV positive field samples which showed positive for VP2 gene was inoculated into eight day old embryonated chicken eggs via yolk sac route and incubated for 12 days. Though there were no appreciable gross changes in the immune organs of the affected embryos, the organs showed positivity for CAV virus by PCR. Histopathologically, the bone marrow revealed depletion of erythroid and myeloid series and thymus showed mild depletion of lymphocytes in the thymic cortex of embryos. In the liver, necrosis and cellular thrombi were present in the parenchyma.
Bone marrow, Chicken infectious anaemia, Egg inoculation, Immunosuppression, PCR, Thymus
