Amelioration of Lipopolysaccharide Induced Acute Lung Injury Using Chlorogenic Acid and Baicalein in Mice: Clinicopathological Study

Lung injury is the most significant cause of morbidity and mortality in humans as well as animals without current effective treatment plan. Lipopolysaccharide (LPS) is pro-inflammatory glycolipid component of cell wall of gram-negative bacteria which lead cause to acute lung injury (ALI). Most prevalent polyphenols in the human diet are chlorogenic acid (CGA), which have a number of biological functions viz anti-bacterial, anti-oxidant and anti-carcinogenic properties. Baicalein (BAC) is a phenolic flavonoid which has been reported to have anti-inflammatory properties. The study aimed to investigate the combined amelio-rative effect of CGA and BAC on LPS induced ALI in murine model. Albino mice were divided into five groups. Seven days prior to instillation of LPS, treatment group mice were administered with CGA and BAC at three different dosages (0.1, 1 and 10 mg/kg body weight) intra-peritoneally. After 24 hours, mice of each group were sacrificed. Samples collected were blood, lungs and BALF. Hb, TLC, DLC estimation (blood); wet to dry weight ratio estimation, histopathology (lungs) and cytology, total protein estimation (BALF) were done. Different doses of CGA and BAC @ 0.1, 1 and 10 mg/kg body weight were studied for ameliorative effect, of which 10 mg/kg body weight was found to be having potential ameliorative effect. CGA and BAC is a rate limiting factors in inflammation and prevents inflammation by reducing the recruitment of neutrophils. Lung injury was amended by CGA and BAC as evident by histopathology in a synergistic manner.