Antioxidant Role of Chlorogenic Acid and Baicalein in Lipopolysac-Charide Induced Acute Lung Injury in Mice

Without an adequate current therapeutic plan, lung injury is the leading cause of morbidity and mortality in both humans and animals. Acute lung injury (ALI) is mostly caused by lipopolysaccharide (LPS), which is a pro-inflammatory glycolipid com-ponent of cell wall of gram-negative bacteria. Chlorogenic acid (CGA) is frequent polyphenol in the human diet with multiple of biological qualities including anti-bacterial, anti-oxidant and anti-carcinogenic capabilities. Baicalein (BAC) is a phenolic flavonoid with anti-inflammatory properties. Thus, the important objective of this study is to investigate the combined amelioratory effect of CGA and BAC on LPS induced ALI Albino mice were divided into five groups named as SHAM/CONTROL, LPS (2 mg/kg body weight), LPS/CGA/BAC/0.1, LPS/CGA/BAC/1 and LPS/CGA/BAC/10 and treatment group mice received intraperitoneal doses of CGA and BAC at three different concentrations (0.1, 1 and 10 mg/kg) seven days prior to the instillation of LPS. In this one-week pre-treatment experiment, mice from each group were scarified after 24 hours. Lungs and BALF were collected. The various biochemical assays (MPO, LPO, NO, SOD and CAT activity), cytokine estimation (TNF-α and IL-6) and immunostaining (i-NOS, Nitrotyrosine and caspase-3) for estimation of inflammation and oxidative stress. CGA and BAC alleviated oxidative stress and inflammation by reducing the production of the inflammatory cytokines. Thus, BAC and CGA had a synergistic effect.