1Department of Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
2Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
*Address for Correspondence Ankita Saini, Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India, E-mail: dr.ankitasaini.as@gmail.com
**Vishal Mahajan, Department of Animal Disease Research Centre, E-mail: mahajanv17@gmail.com
Online Published on 17 September, 2024.
The canine parvovirus enteritis was diagnosed in the present study using the lateral flow assay (LFA), polymerase chain reaction (PCR) for VP-2 gene amplification and fluorescent antibody test (FAT). For this study, faeces samples were taken from 50 canine parvovirus suspected dogs of any breed and sex but age between 3 months to 1 year, presented at Multispeciality Veterinary hospital, GADVASU, Ludhiana from April 2021 to April 2022. Results from LFA, PCR, and FAT revealed that 44% (22/50), 48% (24/50), and 40% (20/50) of canine parvoviral enteritis cases were positive, respectively. In comparison to PCR, ICT and FAT demonstrated 83.3% and 66.7% sensitivity as well as 92.3% and 84.6% specificity, respectively. Further, the canine parvovirus was also identified as CPV-2c using phylogenetic analysis, indicating 100% similarity with QBQ84353.1 strains from China based on a partial VP2 gene. This study demonstrated the field applicability of ICT, a highly sensitive and specific test, and the applicability of FAT, a backup test, in the absence of PCR and ICT.
Canine parvovirus, FAT, ICT, PCR
