Comparison of diagnostic tests for detection of canine parvo viral enteritis

The canine parvovirus enteritis was diagnosed in the present study using the lateral flow assay (LFA), polymerase chain reaction (PCR) for VP-2 gene amplification and fluorescent antibody test (FAT). For this study, faeces samples were taken from 50 canine parvovirus suspected dogs of any breed and sex but age between 3 months to 1 year, presented at Multispeciality Veterinary hospital, GADVASU, Ludhiana from April 2021 to April 2022. Results from LFA, PCR, and FAT revealed that 44% (22/50), 48% (24/50), and 40% (20/50) of canine parvoviral enteritis cases were positive, respectively. In comparison to PCR, ICT and FAT demonstrated 83.3% and 66.7% sensitivity as well as 92.3% and 84.6% specificity, respectively. Further, the canine parvovirus was also identified as CPV-2c using phylogenetic analysis, indicating 100% similarity with QBQ84353.1 strains from China based on a partial VP2 gene. This study demonstrated the field applicability of ICT, a highly sensitive and specific test, and the applicability of FAT, a backup test, in the absence of PCR and ICT.