Cytological diagnosis of seminoma in dog

A 10-year-old intact male Spitz dog was presented at Sanchu Animal Hospital, Chennai for diagnosis and treatment with the history of unilateral scrotal swelling since few months. Physical examination of the affected dog showed a marked enlargement of left testis with peeling of skin due to frequent licking. On palpation of affected testis, mild pain was evinced. No enlargement of regional lymphnodes was noticed. Hematobiochemical studies revealed mild anemia, an increase in serum total protein and globulin and a decrease in A/G ratio. Cytological examination of the aspirated smears from the affected testis was suggestive of seminoma which revealed moderate cellularity with cells being discrete, round to ovoid and exhibiting moderate pleomorphism. The cytoplasm was homogenous and slightly basophilic containing prominent large spherical to ovoid nuclei with coarsely reticular chromatin. In many areas, binucleate and multinucleate cells were present. In addition, mitotic figures were also seen in some areas. Based on the cytological examination, the mass was identified as seminoma.