The Indian Journal of Veterinary Research
Open Access
  • Year: 2010
  • Volume: 18
  • Issue: 2

Molecular detection and differentiation of Mycobacterium tuberculosis complex in human sputum samples using PCR assays: A preliminary report

  • Author:
  • M. Thangaselvam, A. Kidangan, Rishendra Verma, S. P. Ramane
  • Total Page Count: 5
  • Page Number: 50 to 54

Mycobacteria Laboratory, Indian Veterinary Research Institute, Izatnagar-243 122, (Uttar Pradesh).

*Head Division of Biological Standardisation, Indian Veterinary Research Institute, Izatnagar-243 122, (Uttar Pradesh)

Abstract

Thirty five human sputum collected from TB hospital Bareilly were investigated for Mycobacteria based on direct microscopy, culture and by multiplex peR targeting 12.7 kb fragment and IS 611O. DNA wasisolateddirectlyfromsputumsamples. Outof35 samples,25 weresmearpositiveand 18 yielded culture and 16 were positive by the multiplex PeR. 10 samples were negative on smear mircoscopy, culture and PCR.

Keywords

Egg quality, Haugh unit score, Off-flavour, Yolk index, , Yolk colour score