Histoenzymic alterations in buffalo lung due to lead toxicosis: An experimental study

The present study was conducted on six crossbred calves below one year of age. The lead acetate was given @ 25 mg/kg body weight orally for 7 days. After the completion of the experimental period, the lung tissues were collected in liquid nitrogen and cryostat sections of 10 μm thickness were incubated for the demonstration of phosphatases and dehyrogenases. The positive and negative controls were carried out wherever possible. The lungs showed moderate AKPase, ATPase and G-6-Pase activity whereas the SDH and LDH activity varied from weak to moderate in alveolar septae. ACPase activity was increased in histiocytes of alveolar septae. The alveolar ducts showed negligible activity of all the enzymes studied. The results may be correlated with cellular damage and lysis of macrophages of the alveoli.