Antibacterial properties of seabuckthorn (Hippophae rhamnoides L.) leaf extracts against common skin and wound bacteria

The study evaluated in-vitro antibacterial activity of seabuckthorn (Hippophae rhamnoides L.) leaves using disc diffusion method against common skin and wound pathogens. Methanolic hot and cold extracts of green seabuckthorn leaves were tested against 160 microbial isolates obtained from clinical skin and wound infection cases of different animal species. Different concentrations of leaf extracts i.e. 0.5%, 2%, 3%, 4% and 5% were tested against 1×10⁸ cfu/ml bacteria inoculated on Mueller-Hinton agar (MHA). The inhibitory effect of SBT leaf extract at 5% concentration was observed to be almost 50% as compared to the standard drugs used as positive control against isolates.