Latex agglutination test based on the recombinant outer membrane proteins for serodiagnosis of leptospirosis in goats

A total of 281 serum samples collected randomly from goats showing the signs of fever, abortion, repeat breeding and still births as well as from apparently healthy ones were subjected to LAT and MAT based on rLipL32 and rLipL41 antigens. A total of 16 (5.69%) samples were found positive to MAT, whereas rLipL32-LAT and rLipL41-LAT detected 35 (12.45%) and 23 (8.18%) samples as positive, respectively. The sensitivity and specificity of rLipL32-LAT was 87.50% and 92.83%, respectively, while rLipL41-LAT yielded 75.00% and 97.35% sensitivity and specificity, respectively. LAT based on rLipL32 and rLipL41antigens could further be evaluated on a larger number of samples to ensure its utility as a screening test for the sero-epidemiological studies.