Identification and characterization of Mycoplasma synoviae from chicken by Polymerase Chain Reaction (PCR)

A total of 425 samples were collected for the isolation of Mycoplasma synoviae (MS) from chicken from 39 commercial and government poultry farms from different parts of Karnataka, Andhra Pradesh and Tamil Nadu; from both live and dead birds into Frey's mycoplasma broth. We validated and applied polymerase chain reaction (PCR) using primer specific to 16SrRNA gene for the detection of field strains of MS in different poultry farms. Only three field samples yielded amplicon of size 207bp, specific for MS which were further confirmed by sequencing and phylogenic analysis. Overall, the 16SrRNA gene-targeted PCR appeared to be specific, sensitive and a useful tool for the detection of mycoplasmosis causedby MS.