Comparison of monoclonal antibody based latex agglutination test with PCR for diagnosis of Trypanosoma evansi in domestic animals

A cross-sectional study, was conducted to compare, the three diagnostic tests: polymerase chain reaction, (TE-PCR), monoclonal antibody based latex agglutination test (MAb-LAT) and wet blood film (WBF) to detect trypanosomosis in domestic animals from different parts of Haryana, India. A total of 205 animals including cattle (n=88), buffaloes (n=46) and equines (n=71) of Varying age group were randomly sampled for this purpose. TE-PCR could detect 124 samples (60.49%) positive for, Trypanosoma,evansi. (T. evansi) infection and 122 samples (59.51%) using MAb-LAT and 4 samples (1.95%) using WBF. Diagnostic sensitivity(D-SN) and diagnostic specificity (D-SP) values of MAb. LAt using TE-PCR as reference test were 88.43% and 81.48% respectively; DSN and D-SP vallies of TE-PCR were 87.70% and 82.50% respectively using MAb-LAT as reference test Results showed a good correlation between MAb-LAT and TE-PCR. Among the different age group of animals screened, it was found, animals between 1–3 yrs were most susceptible for trypanosomosis and the incidence was more in monsoon and post monsoon, season, MAb-LAT was found to be simple, rapid, convenient, cost-effective test to be used for the field level diagnosis and screenihg of trypanosomosis.