Department of Veterinary Microbiology, G.B. Pant Univ. of Agriculture and Technology, Pantnagar (Uttarakhand)
*Corresponding author: shweta29138@gmail.com
Online published on 3 October, 2018.
The occurrence of four genes, namely, Salmonella invasive (invA), Salmonella enterotoxin (stn), Salmonella fimbrial C (fimC) and Salmonella outer protein B (sopB) genes was investigated by PCR assay in 25 Salmonella cultures isolated from animals and maintained as lyophilized cultures. The invA was present in all the 25 strains, stn gene in 20, fimC gene in 22 sopB gene in 23 Salmonella serovars respectively. Since invA gene was present in all the serovars, it can be a viable target gene for direct detection of Salmonella in clinical samples. Further, the fim operon did not show cross-reactivity with E. coli.
invA, stn, fimC, sopB, PCR, Salmonella