Evaluation of combination of antibiotic with multienzyme preparation on bovine mastitis

The study evaluated the efficacy of multienzyme therapy as an adjunct or alternative to antibiotic therapy in mastitic cows. Twenty one cows affected with clinical mastitis were selected and divided into three treatment groups: Group 1 (n=7) animals were treated with enrofloxacin @ 10 mg/kg b.w, I/M for 5 days. Group 2 (n=7) cows received an intramammary infusion consisting of a mixture of three enzymes viz. chymotrypsin (8 mg), trypsin (8 mg) and papain (4 mg) dissolved in 5 ml NS, at 24 hr. interval for 7 days. Group 3 (n=7) cows received a combination of both parental antibiotic (Enrocin) and intramammary infusion of multienzymes for 7 days at 24 hours interval. Milk samples were collected prior to the treatment (day 1) and on days 4, 7 and 14 post treatment and blood samples on day 1, 7 and 14. There was significant (P<0.05) decrease in the mean serum total protein (TP) and globulin levels in all the treatment groups post treatment as compared to the pretreatment levels while as the mean serum albumin levels and the A: G ratio showed significant (P<0.05) increase post treatment. There was a significant (P<0.05) decrease in the mean total sialic acid (TSA) levels on days 7 and 14 after treatment as compared to the pre-treatment levels in all the three groups. Mean somatic cell count (SCC), standard plate count (SPC) and electrical conductivity (EC) values decreased significantly on days 4, 7 and 14 as compared to that of the day 1. It was concluded that therapeutic intervention with parenteral enrofloxacin and intramammary infusion of proteolytic enzymes improved the udder health of dairy cows affected with clinicalmastitis.