1Doctoral Program of Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia,
2Department of Obstetrics and Gynecology, Faculty of Medicine, Dr Soetomo General Academic Hospital Universitas Airlangga, Surabaya, Indonesia,
3Department of Physiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia,
4Department of Internal Medicine, Division of Metabolic Endocrinology ad Diabetes Dr Soetomo General Academic Hospital, Universitas Airlangga, Surabaya, Indonesia,
5Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia,
*Correspondence: budi.santoso@fk.unair.ac.id
This study aimed to investigate a low-carbohydrate-high protein diet against insulin levels, TNF-α, and p38MAPK expression in PCOS mice model. A total of 18 mice was injected by testosterone propionate injection for 28 days. PCOS mice model was treated with a low carbohydrate and high protein diet for 20 days. Insulin and TNF-α levels from blood serum were measured by the enzyme-linked immunosorbent assay (ELISA). The p38MAPK expression in ovarian tissue was evaluated by immunohistochemistry (IHC). There was significantly decreased of insulin and TNF-α levels in the PCOS mice model with a low carbohydrate and high protein diet for 20 days (p< 0.001). In addition, this diet was not statistically induced p38MAPK activity. A significant decreased of insulin and TNF-α levels after a low carbohydrate and high protein diets in the PCOS mice model. Our findings highlight the low carbohydrate and high protein diets useful to reduce PCOS progression with obesity.
Diet, Insulin, Low, PCOS mice model, p38MAPK activity, TNF-α
