Assessment of vitamin E as an anti-oxidant in pre and postoperative treatment schedule of buffaloes subjected to transabdominal diaphragmatic herniorrhaphy

The present study was aimed at evaluation of therapeutic effects of a-tocopheryl acetate and sodium selenite combination as an anti-oxidant in the treatment schedule of diaphragmatic hernia affected buffaloes subjected to transabdominal diaphragmatic herniorrhaphy. Comparisons were made with the base values and also with the affected buffaloes subjected to trans-abdominal diaphragmatic herniorrhaphy without any anti-oxidant therapy (diseased control group, n=10). The mean base values of oxidative stress parameters in blood viz. MDA, GSH, OSF and serum copper levels were significantly higher, whereas serum zinc level was lower in all the affected than in healthy farm buffaloes. In diseased control group, significant rise in oxidative stress as evident by rise in mean values of GSH (27%), MDA (22%), PCV (3%), OSF (50%) and copper (120%), and significant reduction in serum zinc concentration (46%) were observed as compared to base values at 48 h intervalpost-herniorrhaphy. In treatment group (n=10), administration of 10 mL of E-care Se containing a-tocopheryl acetate 550IU and sodium selenite 15 mg, intramuscularly just after laparorumenotomy, prior to herniorrhaphy and then at 24 h and 48 h intervals post-herinorrhaphy resulted in the reduction in OSF (28%) and GSH (45%), without any appreciable change in MDA and significant increase in serum zinc concentration at 48 h interval.