Assessment of ascorbic acid as an anti-oxidant in buffaloes during diaphragmatic herniorrhaphy

The present study was aimed to evaluate the therapeutic effects of ascorbic acid as an anti-oxidant in the treatment schedule of diaphragmatic hernia affected buffaloes subjected to trans-abdominal diaphragmatic herniorrhaphy. Comparisons were made with the index values from healthy farm buffaloes (n=8), base values of treated (treatment group, n=10) and also with the untreated affected buffaloes (disease control group, n=10) subjected to trans-abdominal diaphragmatic herniorrhaphy. The mean base values of oxidative stress parameters in blood viz. MDA, GSH, OSF and serum copper levels were significantly higher, whereas serum zinc level was lower in all affected, than healfhy farm buffaloes. In treatment group administration of ascorbic acid (@ 7.5 g/L dextrose isotonic saline, iv) in pre- and post-operative treatment schedule, reduced the MDA (3.77%), GSH (27.04%) and OSF (20%) at 48 hr interval. Whereas, in diseased control group, significant rise-in GSH (27%), MDA (22%), PCV (3%), OSF (SO%), copper (120%) and significant reduction in serum zinc concentration (46%) were observed as compared to base values at 48 hr interval.