1Department of Surgery and Radiology G.B. Pant University of Agriculture and Technology, Pantnagar-263145 (Uttaranchal)
2Department of Microbiology G.B. Pant University of Agriculture and Technology, Pantnagar-263145 (Uttaranchal)
3College of Veterinary and Animal Sciences G.B. Pant University of Agriculture and Technology, Pantnagar-263145 (Uttaranchal)
The present study was conducted on 10 male buffalo calves of 18–24 months of age, with the damage in the SDF tendon, to assess the regenerating capability of cultured tenoblasts in the repair of injured tendon. For in vitro cultivation, the parent tenoblasts were harvestedfrom epitenon of Superficial Digital Flexor Tendon (SDFT) collected from cadaveric buffalo calves from slaughter house immediately after salughter of animals. Tenoblasts were cultured in MEM culture medium supplemented with 10% FCS and ascorbic acid (@10μg/mL) and incubated at 37°C in CO2 incubator with 5% CO2. When the cells reached confluence, they were transplanted in the damaged tendon in 5 animals (group A), while in other 5 animals only epitenon was sutured and the gap was filled with blood clot, which served as control (group B). The efficiency oftenoblast transplantation for regeneration of damaged tendon was evaluated by fasciagraphy and histopathological studies for a period of 60 days post-transplantation. Tenograms revealed comparatively more adhesions in control group than in transplanted group. Histopathological observations revealed more cellular reactions and early healing of the gap in transplanted group of animals, while control (group B) animals revealed immature collagen, which is oriented in different directions. The study indicated that tenoblasts could be harvestedfrom the epitenon of SDFT and could be used for regeneration of damaged tendon.
Buffalo, Tendon repair, Tenoblast
