Intra-abdominal laparoscopic vasectomy was performed in 5 clinically healthy, adult male dogs under xylazine-ketamine anaesthesia. Animals were placed in Trendelenburg position and insufflation of peritoneum was achieved by CO2 (2 litre/minute) at 10 mmHg pressure gradient. The monopolar scissors were used for cauterization as well as for cutting the vas deferens by applying 60 W monopolar coagulation current. Clinical observations revealed no significant change in heart rate and respiration rate. Differential leukocyte count revealed significant neutrophilia and corresponding lymphopenia on 3rd postoperative day. Significant increase (P<0.05) in plasma alkaline phosphatase and acid phosphatase levels was observed on day 3 postoperatively. The oxidative stress to the animals was estimated by measuring the indicators of oxidative stress, like lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD), reduced glutathione activity and acute phase protein-ceruloplasmin in plasma. Increase in LPO was non-significant while the activity in CAT increased significantly (P<0.05) at days 1 and 3 postoperatively. SOD significantly reduced (P<0.05) immediately after operation while reduced glutathione activity recorded was significantly higher immediately after operation and at day 1 postoperatively. The acute phase protein-ceruloplasmin level in plasma was slightly increased (P>0.05) after surgery. Changes in plasma cortisol and testosterone levels were non significant during postoperative period.
