Studies on MEM-Eagle, M-199 and Ham's F-12 culture media for in-vitro cultivation of bovine tenoblasts

Tenoblasts for culture were harvested from the epitenon of superficial digital flexor tendon of a young buffalo calf and cultured in three commercially available growth media viz. MEM-Eagle, M199 and Ham's F-12 supplemented with 10% fetal calf serum (FCS) and ascorbic acid @ 100 μg/ml. The media used for replenishment of growth medium during the process of incubation were supplemented with 5% FCS. After tenoblasts inoculation, culture media were examined daily for any change in pH, culture bottles were examined under inverted microscope on 5^th, 12^th, 19^th and 28^th day post-culture to see cell proliferation. Bovine tenoblasts exhibited cellular growth of varying extent in MEM-Eagle growth medium, while poor cell proliferation was observed in M-199, and no growth in Ham's F-12 medium on various intervals of observation.