Evaluation of healing potential of autologous bone marrow-derived nucleated cells on incisional wounds in dogs

The study was conducted on 14 dogs divided into three groups, BI, BT and PI. In animals of groups BI and BT (n=5 each) the incisional wounds were treated by autologous bone marrowderived nucleated cells injected into sutured wound margins and applied topically over the sutured skin edges, respectively. Wounds in the animals of PI group were dressed with 5% povidone-iodine solution (control). Efficacy of treatment was evaluated by physiological parameters, haemoglobin concentration, differential leukocyte count, plasma glucose, total protein and albumin, quantity and quality of exudates, extent of peripheral swelling, wound microbiology and duration of complete healing. There was no significant (P>0.05) difference between the different treatment groups with regard to physiological and haematobiochemical parameters, extent and type of exudate and peripheral swelling. Duration of complete healing was, however, significantly (P<0.05) lesser in BT than in BI and PI groups. It was concluded that application of bone marrow derived nucleated cells can help healing of incisional wounds.