Application of mesenchymal stem cells for surgical and chronic wound healing in dogs

In the present study, 5 cases of wounds were treated with bone marrow derived stem cells. Treated wounds included contaminated open wound, and clean incised wound along with control category. After aseptic collection of bone marrow from the iliac crest of the dog, MSCs were isolated after washing with Dulbecco's phosphate buffered saline and sub culturing it using standard protocol. Then a definite number (1 x 106) of MSCs were suspended in phosphate buffer saline and used for transplantation. The wound site was prepared aseptically and MSCs were injected at wound margins subcutaneously. A maximum of three doses at weekly intervals were used in case of contaminated wound to hasten the healing process. The treated wounds were evaluated by clinical observation at regular intervals till the completion of healing. The results showed that stem cell therapy resulted in faster healing in comparison to the conventional wound dressings.