1PhD Scholar, MVSc Scholar, Department of surgery & Radiology, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar, 263145 (Uttarakhand)
2Dean, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar, 263145 (Uttarakhand)
3Assistant professor, Department of MBGE, G.B. Pant University of Agriculture & Technology, Pantnagar, 263145 (Uttarakhand)
Online published on 1 March, 2021.
MSCs are proven candidate for cell therapy, their isolation and in vitro proliferation is challenging. They represent only one in 10,000 nucleated cells in bone marrow of healthy individuals. So the isolation process needs to be standardized in order to extract good cell yield. The present study was conducted to standardize the isolation protocol of bone marrow derived MSCs along with their in- vitro differentiation into osteoprogenitor cells followed by its characterization. It was concluded that MSCs were isolated utilizing Ficoll- Paque density gradient centrifugation technique showed satisfactory yield. Differentiated cells characterized utilizing Alizarin Red S staining showed stain mineral deposition by developing osteoblasts.
Alizarin red S stain, Mesenchymal stem cells, Osteoprogenitor cells, Rabbit
