INROADS- An International Journal of Jaipur National University

  • Year: 2013
  • Volume: 2
  • Issue: 1

Simultaneous Estimation of Lamivudine and Stavudine by using Isocratic Reverse Phase High Performance Liquid Chromatography Method

School of Basic Sciences, Department of Chemistry, Jaipur National University, Jaipur, Agra By Pass, Near New RTO office, Jagatpura, Jaipur 302017, Rajasthan, India

Abstract

A simple, precise, fast Reversed Phase High Performance Liquid Chromatographic (RP-HPLC) assay method by external standard method was developed for the determination of Lamivudine and Stavudine in a solid dosage form. The chromatographic separation was performed using Phenomenex Bondalone 300 x 3.90 mm, 10 μ column. Analytes were resolved by using mobile phase 5:95 mixture of Acetonitrile: water at UV 265 nm as detection wavelength.

Keywords

Lamivudine, Stavudine, High Performance Liquid chromatography