Synchronization of estrus and ovulation for fixed time insemination in buffaloes under field conditions during summer season

The study was planned to induce estrus in true anoestrus buffaloes under field conditions for fixed time insemination. The animals (buffaloes and buffalo heifers, n=6 each) were selected from different villages adopted by CIRB under field progeny testing program. All animals received 500 mg progesterone on day 0 followed by 500 units PMSG and 25 mg prostaglandin, I/M on day 4. All animals were inseminated once on day 7 using frozen thawed semen. Scanty to clear mucus discharge was observed 55–65 hrs after prostaglandin injection in all animals. Behavioral signs (bellowing) were observed only in two buffaloes. Per-rectal examination revealed partial to complete relaxation of cervix in 10 animals. Three buffaloes and 2 heifers conceived following fixed time AI to the above treatment. The findings suggest that above treatment can be used during summer season to induce cyclicity in anoestrus buffaloes.