INTAS POLIVET
Open Access
  • Year: 2015
  • Volume: 16
  • Issue: 2

Molecular Diagnosis and Therapeutic Management of Black Quarter-A Clinical Study of 21 Bovines

  • Author:
  • A.V. Bhonsle1,, A.U. Bhikane2, M.A. Khan3, M.B. Kulkarni4, A.G. Karpe5
  • Total Page Count: 4
  • Page Number: 376 to 379

1Assistant Professor, Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University (MAFSU), Udgir-413517, Dist. Latur, Maharashtra

2Associate Professor, Dept. of Veterinary Medicine, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University (MAFSU), Udgir-413517, Dist. Latur, Maharashtra

3Assistant Professor, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University (MAFSU), Udgir-413517, Dist. Latur, Maharashtra

4Laboratory Technician, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University (MAFSU), Udgir-413517, Dist. Latur, Maharashtra

5Dean, Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University (MAFSU), Udgir-413517, Dist. Latur, Maharashtra

Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Maharashtra Animal and Fishery Sciences University (MAFSU), Udgir-413517, Dist. Latur, Maharashtra

*Corresponding author. E-mail: ashokvbhonsle@hotmail.com

Online published on 2 April, 2016.

Abstract

Twenty one clinically diagnosed bovines were identified as Clostridium chauvoei infection by molecular method. Bacterial isolation yielded 28 isolates including Clostridium chauvoei, Clostridum tertium and Clostridium sporogenes. The polymerase chain reaction (PCR) was used to amplify specific segments of the Ccta and 16S ribosomal RNA gene of Clostridium chauvoei, a major pathogen of ruminants. The extracted DNA from bacterial isolates was quantitated and used as a template for PCR. The animals were treated with antibiotics, corticosteroids, NSAIDS, antihistaminic and intensive fluid therapy successfully because of timely treatment.

Keywords

Black quarter, buffalo, cattle, Clostridium chauvoei, polymerase chain reaction