2Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Bareilly
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry Odisha University of Agriculture and Technology (OUAT), Bhubaneswar-751003 (Odisha)
*Corresponding author. E-mail: dearlita123@gmail.com
Online published on 2 April, 2016.
Bacterial isolates from prolonged non-healing wounds in canines were isolated and screened for their biofilm producing ability. Antibiotic sensitivity testing for both biofilm forming and planktonic forms of selected isolates of Staphylococcus aureus (n=7), E. coli (n=5) and P. aeruginosa (n=4) was performed. 0.3% Bentonite clay was adopted as a substrate along with MHA for biofilm forming bacterial isolates. The results showed that average zone of inhibition and resistance against antibiotics was higher for biofilm forming bacterial isolates than their planktonic counterparts. Therapeutic resistance is much higher for bacteria that is commonly observed in nutrient agar media during screening for antibiotic sensitivity testing, suggesting there is a need for use of other substrates.
Antibiotic resistance, bentonite clay, biofilm, canine, wound
