1Assistant Research Scientist, Livestock Research Station, NAU, Navsari
2Professor and Head, Livestock Research Station, NAU, Navsari
3Retired Professor and Head, Department of Veterinary Public Heath, Livestock Research Station, NAU, Navsari
Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Anand Agricultural University (AAU), Anand-388001, Gujarat
*Corresponding author. E-mail: drmanish911@yahoo.com
Online published on 2 April, 2016.
An investigative study on mycotic causes of sub-clinical intramammary infections (IMI), antifungal drug sensitivity and in-vivo use of Nystatin was carried in crossbred cows. Total 184 quarter milk samples from 46 lactating crossbred cows were screened for presence of sub-clinical intramammary infections and identification of causal organisms. Of 32 fungal isolates identified, twelve (37.50%) were recovered as mono-microbic cause. Whereas, twelve isolates (37.50%) were recovered from mixed infections with bacteria and remaining 8 isolates with mixed infections of moulds. Total 20 different fungal isolates were subjected to in-vitro antifungal drug sensitivity against Amphotericin-B, Clotrimazole, Nystatin and Fluconazole. Based on sensitivity, 50, 000 IU Nystatin in 10 ml of sterile distilled water was administered into 8 selected quarters with fungal mastitis once daily for five consecutive days. Seven quarters did not showed any growth in quarter milk samples collected 15 days after last infusion indicating 87.50% efficacy of Nystatin in fungal sub-clinical intramammary infections.
Infection, intramammary, mycotic, nystatin, sub-clinical, yeast
