Antibiogram of Bacterial Isolates from Cervico-Vaginal Mucus (CVM) of Endometritic Cows

The study evaluates antibiotic sensitivity pattern of different bacterial flora isolated from cervico-vaginal mucus (CVM) collected from cows suffering with endometritis. Overall 563 cervico-vaginal discharges were collected, 136 samples were free from bacterial growth and 427 exhibited bacterial growth in nutrient broth. In-vitro antibiotic sensitivity test for each turbid sample was done by disc diffusion method and was spread on Mueller-Hinton agar (MHA) along with 14 different antibiotic discs. Enrofloxacin (n=427) was highly sensitive against 72.4 percent isolates whereas 21.0 percent isolates were moderately sensitive and 6.6 percent were resistant. For Ciprofloxacin (n=427), the sensitivity levels recorded were 71.9 percent as highly sensitive, 21.5 percent as moderately sensitive and 6.6 percent as resistant. Similarly for Gentamicin (n=405), isolates exhibited sensitivity levels as highly sensitive, moderately sensitive and resistant in 27.6, 62.0 and 10.4 percent, respectively. Isolates exhibited sensitivity as highly sensitive, moderately sensitive and resistant in 57.4, 29.9 and 12.7 percent respectively, for Ofloxacin (n=425); 16.3, 31.7 and 52.0 percent for Amoxicillin (n=426); 5.6, 10.8, and 83.6 percent for Penicillin (n=409); 0, 7.3 and 92.7 percent for Metronidazole (n=289); 32.7, 48.2 and 19.1 percent isolates for Ceftriaxone (n=309); 26.0, 37.4 and 39.5 percent isolates for Cephalexin (n=215); 23.8, 42.4 and 33.8 percent for Oxytetracycline (n=269); 11.1, 54.2 and 34.7 percent for Streptomycin (n=216); 6.5, 17.5 and 76.0 percent for Cloxacillin (n=217); 22.2, 27.3 and 50.5 percent for Ampicillin (n=216) and 68.1, 19.0 and 12.9 percent isolates for Levofloxacin (n=216), respectively. The highest sensitivity was recorded for Enrofloxacin followed by Ciprofloxacin and Levofloxacin whereas highest resistance was recorded for Metronidazole followed by Penicillin and Amoxicillin.