Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar-243 122, India.
*Corresponding author, Tel: +91-581-2303382, Fax: +91-581-2303284, E-mail: subodh@ivri.up.nic.in, subkum@gmail.com
A fragment of 151 bp corresponding to exon-5 of ITGB1 gene was amplified in 45 buffaloes (Bubalus bubalis) of murrah breed. The RFLP analysis showed the absence of polymorphism in this fragment with respect to TruII restriction enzyme and showed only one restriction site, which produced two fragments of 21 and 130 bp. The putative metal ion binding D-X-S-X-S sequence motif in the I-like domain of b1 subunit was highly conserved in these buffaloes. Three and nine nucleotide sequence variations were found in buffalo sequences when compared to that of cattle and human, respectively. The sequence of the amplicon which was the first report on buffalo ITGB1, was submitted to GenBank.
Buffalo, exon-5, integrin β−1, murrah, Tru1I, RFLP
