1Division of Virology, Indian Veterinary Research Institute, Mukteshwar-263 138, India.
2Veterinary College, Punjab Agricultural University, Ludhiana-141004, India.
*Corresponding author: Tel: +91-5942-286348; Fax: +91-5942-286347; E-mail: bhanu6467@scientist.com
An indirect ELISA was optimized and evaluated using purified goat pox virus (GPV, cell culture attenuated) antigen and different groups of serum samples from goats with known and unknown immune status for the detection of GPV antibodies. The cut-off value higher by 0.2 at A492 of the control negative sera gave 90% sensitivity and 94.26% specificity for the assay. Of the total 1708 goat serum samples screened, 1054 (61.70%) were positive in indirect ELISA, where as only 219 (12.82%) samples were positive in counterimmunoelectrophoresis (CIE) test, a preliminary diagnostic test for goat pox. The indirect ELISA was observed to be superior to CIE test in the detection of GPV antibodies in the goat sera and its use has been recommended.
Antibody, counter immunoelectrophoresis, goat poxvirus, indirect ELISA
