Genetic diversity between red jungle fowl and domestic chicken for BL B2 region

Genetic polymorphism in BL B2 gene between Red Jungle Fowl (RJF) and domesticated chicken through sequence homology comparisons was studied. A 277 bp amplicon representing second exon corresponding to β1 extra cellular domain of BL B2 gene was amplified using chicken specific primers and sequenced (Accession No DQ885564). Sequence homology comparisons of the 277 bp sequence of RJF showed that it had the maximum genetic similarity (93.3%) with BW1 haplotype of RJF. Among the chicken B haplotypes, B21 haplotype, irrespective of its genetic background i.e. either it is from White Leghorn or Thai native chicken, showed maximum sequence homology. Among chicken breeds, RJF showed more genetic similarity with Chinese and Korean native in comparison to Indian native chicken breed.