Journal of Applied Animal Research
  • Year: 2007
  • Volume: 31
  • Issue: 1

Molecular characterization of ompA gene of Pasteurella multocida serotype B: 2

  • Author:
  • S. Singh, V.P. Singh, A.A. Kumar, S. Joseph, P.S. Cheema, S.K. Srivastava, Nem Singh
  • Total Page Count: 4
  • Page Number: 97 to 100

Division of Bacteriology and Mycology Indian Veterinary Research Institute Izatnagar-243122, India.

*For correspondence: E-mail: vijendra@ivri.up.nic.in

Abstract

The ompA gene of Pasteurella multocida serotype B: 2 (strain P52) was cloned in InsT/A vector for sequencing and gene sequence was compared with that of other strains (232, 95010872, T94289 and 95120769) of serogroup A. It revealed a 1077 nucleotide and it had additional 30 bp than that of serotype A: 3. About 11.5 to 14% divergence in ompA gene sequence was observed between serotype B: 2 and various strains of serogroup A of P. multocida. A variation of about 20% in the amino acid sequence of outer membrane protein A (OmpA) was also recorded between serogroups A and B.

Keywords

Cloning, Pasteurella multocida, outer membrane protein A (OmpA)