Journal of Applied Animal Research

  • Year: 2007
  • Volume: 32
  • Issue: 1

Comparison of method of application on the effect of amylolytic enzymes on In vitro ruminal starch digestion

  • Author:
  • R. Rojo1, G.D. Mendoza2, F.X. Plata2, A. Lara3, R. Bárcena4
  • Total Page Count: 4
  • Page Number: 81 to 84

1Universidad Autónoma del Estado de México México.

2Universidad Autónoma Metropolitana, Unidad Xochimilco Departamento de Produccción, Agricola y Animal, 04960 México D.F.

3Universidad Autónoma Chapingo Estado de México, 56230.

4Colegio de Postgradados, Campus Montecillo Estado de México 56230.

*For correspondence: Fax: (52) 5959520200; E-mail: gmendoza@correo.xoc.uam.mx

Abstract

An in vitro experiment was designed to study the efficacy of two amylolytic enzymes on starch digestion of corn applied to grain 24 h before incubation or at the beginning of the fermentation. Both enzymes, amylase from B. licheniformis and glucoamylase from A. niger, increased starch digestion and there was no effect of the aspersion of the enzyme before the incubation, or the addition of the solution during the fermentation. It is concluded that even when enzymes are soluble proteins, resistance to ruminal degradation is not improved by the contact with the grain before incubation.

Keywords

Amylase, glucoamylase, corn, exogenous enzymes, in vitro digestibility