Journal of Applied Animal Research
  • Year: 2007
  • Volume: 32
  • Issue: 1

A PCR-RFLP method for specific detection of Theileria annulata

  • Author:
  • R. Ravindran, B.C. Saravanan, J.R. Rao, A.K. Mishra, G.C. Bansal, D. Ray
  • Total Page Count: 2
  • Page Number: 111 to 112

Division of Parasitology, Indian Veterinary Research Institute Izatnagar-243 122, India.

*Corresponding author: College of Veterinary and Animal Sciences, Pookot, Lakkidi, Wayanad-673576, India; Tel: +919447713422; E-mail: drreghuravi@yahoo.com

Abstract

A polymerase chain reaction amplifying the ribosomal RNA gene from the genomic DNA was standardized and it amplified monomorphic ~564 bp product from B. bigemina, T. annulata and host leucocyte genomic DNA templates. Use of the enzyme kpnI resulted in restriction digestion only with T. annulata specific PCR product yielding 466 and 98 bp fragments.

Keywords

PCR-RFLP, Theileria annulata, bovines