Molecular Genetics Laboratory, Central Avian Research Institute, Izatnagar-243 122, India
*For correspondence: E-mail: skgcari@yahoo.co.uk
1Part of M.V.Sc. dissertation work of first author, IVRI deemed university, Izatnagar, India.
DNA polymorphism of IL-2Rγ and IL-15Rα was studied in 70 birds belonging to third generation of specialized White Leghorn chicken lines, divergently selected for humoral immune response to sheep erythrocytes by PCR-RFLP technique using specific primers. Amplification revealed product of 600bp for IL-2Rγ and 246bp for IL-15Rα genes. These amplicons digested with Hph I and Alu I revealed similar and monomorphic patterns in specialized layer chicken lines. It may be inferred that although specific primers amplified the target gene sequence but the PCR-RFLP pattern varied under different genetic backgrounds.
Nucleotide polymorphism, immune response, chicken, PCR, RELP
