1Department of Urology, Shaheed Faghihi Hospital, Iran
2Department of Obstetrics and Gynecology, Shaheed Faghihi Hospital, Iran
3Department of Pathology, Nemazee Hospital, Iran
4Gastroenterohepatology Research Center, Nemazee Hospital, Iran
5Experimental Pathology Research Center, Shiraz University of Medical Sciences, Shiraz, 71345-1744, Iran
*Corresponding author: Tel: +98-711-2351089-97; Fax: +98-711-6256051; E-mail: pmasoudi@hotmail.com
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To evaluate the use of fresh placental membrane as a feasible, economic and acceptable organic agent for bladder reconstruction and replacement, twelve cross hybrid dogs underwent partial cystectomy and subsequent closure (two controls) or patch augmentation with a 4×4 centimeter patch of human placental membrane, sutured to the remaining bladder in a watertight fashion. The dogs were sacrificed six weeks after surgery. Grafts remained in place in all cases, except in one of the dogs in the augmentation group which developed patch perforation, urine leakage and finally peritonitis. In others, histological examinations revealed evidence of regeneration of normal-appearing urothelium, lamina propria, neovascularization, retracting placental patch, and reconstitution of a normal-appearing and functioning bladder. This showed that placental membranes, because of their low antigenic properties, easy availability and tolerability by host urinary tract, provide an excellent graft material for the urinary tract reconstructions.
Bladder reconstruction, placental membrane, graft
