1Department of Animal Science, College of Agriculture, Ataturk University, 25240, Erzurum, Turkey
2Department of Horticulture, College of Agriculture, Ataturk University, 25240, Erzurum, Turkey
*Corresponding author: Tel: 904422312573; Fax: 904422360938; E-mail: ozdemirm@atauni.edu.tr
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method has been used to detect polymorphism in Kappa-casein gene in cattle. A 351 bp Kappa-casein gene fragment was amplified and the PCR products were digested with four different restriction enzymes i.e. Alu I, Hind III, Hinf IandTaq I. All these enzymes were able to distinguish between Kappa-casein A and B types, however Hind III, Hinf I and Taq I were found more effective for kappa-casein variants than Alu I.
Kappa-casein, PCR-RFLP, genetic polymorphism
